eprintid: 1825
rev_number: 10
eprint_status: archive
userid: 6
dir: disk0/00/00/18/25
datestamp: 2013-10-04 10:29:32
lastmod: 2014-12-05 09:20:01
status_changed: 2013-10-04 10:29:32
type: article
metadata_visibility: show
creators_name: Morrison, Greg
creators_name: Hyeon, Changbong
creators_name: Hinczewski, Michael
creators_name: Thirumalai, D.
creators_id: greg.morrison@imtlucca.it
creators_id: 
creators_id: 
creators_id: 
title: Compaction and tensile forces determine the accuracy of folding landscape parameters from single molecule pulling experiments
ispublished: pub
subjects: QC
divisions: EIC
full_text_status: public
keywords: PACS: 87.10.-e, 87.15.Cc, 87.64.Dz, 87.80.Nj

note: © 2011 American Physical Society
abstract: We establish a framework for assessing whether the transition state location of a biopolymer, which can be inferred from single molecule pulling experiments, corresponds to the ensemble of structures that have equal probability of reaching either the folded or unfolded states (Pfold=0.5). Using results for the forced unfolding of a RNA hairpin, an exactly soluble model, and an analytic theory, we show that Pfold is solely determined by s, an experimentally measurable molecular tensegrity parameter, which is a ratio of the tensile force and a compaction force that stabilizes the folded state. Applications to folding landscapes of DNA hairpins and a leucine zipper with two barriers provide a structural interpretation of single molecule experimental data. Our theory can be used to assess whether molecular extension is a good reaction coordinate using measured free energy profiles.
date: 2011-03
date_type: published
publication: Physical Review Letters
volume: 106
publisher: American Physical Society
pagerange: 138102
id_number: 10.1103/PhysRevLett.106.138102
refereed: TRUE
issn: 0031-9007
official_url: http://link.aps.org/doi/10.1103/PhysRevLett.106.138102
related_url_url: http://arxiv.org/abs/1103.0425
citation:   Morrison, Greg and Hyeon, Changbong and Hinczewski, Michael and Thirumalai, D.  Compaction and tensile forces determine the accuracy of folding landscape parameters from single molecule pulling experiments.  Physical Review Letters, 106.  p. 138102.  ISSN 0031-9007      (2011)  
document_url: http://eprints.imtlucca.it/1825/1/11MorrisonPRL.pdf