eprintid: 3163
rev_number: 7
eprint_status: archive
userid: 69
dir: disk0/00/00/31/63
datestamp: 2016-02-29 10:22:42
lastmod: 2016-02-29 10:22:42
status_changed: 2016-02-29 10:22:42
type: conference_item
metadata_visibility: show
creators_name: Iacopetti, Paola
creators_name: Tarantino, I.
creators_name: Cozza, Arianna
creators_name: Santi, S.
creators_name: Savarino, G.
creators_name: Fogli, Stefano
creators_name: Pietrini, Pietro
creators_name: Pellegrini, Silvia
creators_id: 
creators_id: 
creators_id: 
creators_id: 
creators_id: 
creators_id: 
creators_id: pietro.pietrini@imtlucca.it
creators_id: 
title: A flexible SNP genotyping system to study allelic determinants of brain function
ispublished: pub
subjects: RC0321
divisions: CSA
full_text_status: none
pres_type: paper
note: Abstract published in American Journal of Medical Genetics Part B: Neuropsychiatric Genetics, Special Issue: XIV World Congress on Psychiatric Genetics, 5 October 2006,
Volume 141B, Issue 7
abstract: We developed a system for SNP-genotyping consisting of two ‘‘microchips’’, one investigating 40 SNPs in genes involved in the hepatic metabolism of antidepressant drugs, the other 100 SNPs in genes involved in brain response to antidepressants. Candidate genes were chosen by literature screening. SNPs were selected on HapMap data, using the pairwise algorithm combinedwith the ‘‘BestN’’ method; a few SNPs, reported as associated with treatment outcome, were added to the set. The main advantages of our experimental design are: a) simultaneous analysis of several genes (22) involved in distinct aspects of antidepressant response; b) a comprehensive search for common variability in candidate genes by HapMap ‘‘tag-SNPs’’. DNA samples are genotyped by ‘‘padlock probes’’, which specifically recognise the two variants of each SNP; padlock probes are amplified with universal primers, and contain unique tags for hybridisation to known positions on ad hoc designed micro-arrays, where the two alleles are distinguished by distinct fluorophores. Since reactions carried out on multiplex and signal intensities on microarrays are homogeneous, as they are not affected bySNPflanking sequences, tens of SNPsfrom tens of individuals can be analysed simultaneously on a single slide, with high accuracy and reproducibility. Considering that 100 SNPs from manygenes related to neural transmission, plasticity and neurogenesis are included in the secondmicrochip and that each of them can easily be substituted withothers if needed, our system represents a powerful tool for investigating genetic influence on normal and pathological brain function.
date: 2007
date_type: published
publisher: Wiley
event_title: 14th World Congress on Psychiatric Genetics, October 28th–November 1st, 2006
event_location: Cagliari, Italia
event_dates: October 28 - November 1, 2006
event_type: conference
refereed: TRUE
book_title: Book of abstracts of the 14th World Congress on Psychiatric Genetics
official_url: http://onlinelibrary.wiley.com/doi/10.1002/ajmg.b.v141b:7/issuetoc
citation:   Iacopetti, Paola and Tarantino, I. and Cozza, Arianna and Santi, S. and Savarino, G. and Fogli, Stefano and Pietrini, Pietro and Pellegrini, Silvia  A flexible SNP genotyping system to study allelic determinants of brain function.  In: 14th World Congress on Psychiatric Genetics, October 28th–November 1st, 2006, October 28 - November 1, 2006, Cagliari, Italia         (2007)