TY  - JOUR
VL  - 35
SP  - 92 
PB  - Elsevier
A1  - Cristallini, Caterina
A1  - Rocchietti, Elisa Cibrario
A1  - Accomasso, Lisa
A1  - Folino, Anna
A1  - Gallina, Clara
A1  - Muratori, Luisa
A1  - Pagliaro, Pasquale
A1  - Rastaldo, Raffaella
A1  - Raimondo, Stefania
A1  - Saviozzi, Silvia
A1  - Sprio, Andrea E.
A1  - Gagliardi, Mariacristina
A1  - Barbani, Niccoletta
A1  - Giachino, Claudia
JF  - Biomaterials
IS  - 1
Y1  - 2014///
EP  -  104
ID  - eprints3250
N2  - Abstract Despite the enormous progress in the treatment of coronary artery diseases, they remain the most common cause of heart failure in the Western countries. New translational therapeutic approaches explore cardiomyogenic differentiation of various types of stem cells in combination with tissue-engineered scaffolds. In this study we fabricated PHBHV/gelatin constructs mimicking myocardial structural properties. Chemical structure and molecular interaction between material components induced specific properties to the substrate in terms of hydrophilicity degree, porosity and mechanical characteristics. Viability and proliferation assays demonstrated that these constructs allow adhesion and growth of mesenchymal stem cells (MSCs) and cardiac resident non myocytic cells (NMCs). Immunofluorescence analysis demonstrated that stem cells cultured on these constructs adopt a distribution mimicking the three-dimensional cell alignment of myocardium. qPCR and immunofluorescence analyses showed the ability of this construct to direct initial {MSC} and {NMC} lineage specification towards cardiomyogenesis: both {MSCs} and {NMCs} showed the expression of the cardiac transcription factor GATA-4, fundamental for early cardiac commitment. Moreover {NMCs} also acquired the expression of the cardiac transcription factors Nkx2.5 and {TBX5} and produced sarcomeric proteins. This work may represent a new approach to induce both resident and non-resident stem cells to cardiac commitment in a 3-D structure, without using additional stimuli.
SN  - 0142-9612
UR  - http://www.sciencedirect.com/science/article/pii/S0142961213011587
KW  - Biocompatibility; Cardiac tissue engineering; Cell morphology; ECM (extracellular matrix); Scaffold; Stem cell
TI  - The effect of bioartificial constructs that mimic myocardial structure and biomechanical properties on stem cell commitment towards cardiac lineage
AV  - none
ER  -