eprintid: 3250
rev_number: 6
eprint_status: archive
userid: 69
dir: disk0/00/00/32/50
datestamp: 2016-03-21 09:35:11
lastmod: 2016-03-21 09:35:11
status_changed: 2016-03-21 09:35:11
type: article
metadata_visibility: show
creators_name: Cristallini, Caterina
creators_name: Rocchietti, Elisa Cibrario
creators_name: Accomasso, Lisa
creators_name: Folino, Anna
creators_name: Gallina, Clara
creators_name: Muratori, Luisa
creators_name: Pagliaro, Pasquale
creators_name: Rastaldo, Raffaella
creators_name: Raimondo, Stefania
creators_name: Saviozzi, Silvia
creators_name: Sprio, Andrea E.
creators_name: Gagliardi, Mariacristina
creators_name: Barbani, Niccoletta
creators_name: Giachino, Claudia
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creators_id: mariacristina.gagliardi@imtlucca.it
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title: The effect of bioartificial constructs that mimic myocardial structure and biomechanical properties on stem cell commitment towards cardiac lineage
ispublished: pub
subjects: QD
subjects: RZ
divisions: CSA
full_text_status: none
keywords: Biocompatibility; Cardiac tissue engineering; Cell morphology; ECM (extracellular matrix); Scaffold; Stem cell
abstract: Abstract Despite the enormous progress in the treatment of coronary artery diseases, they remain the most common cause of heart failure in the Western countries. New translational therapeutic approaches explore cardiomyogenic differentiation of various types of stem cells in combination with tissue-engineered scaffolds. In this study we fabricated PHBHV/gelatin constructs mimicking myocardial structural properties. Chemical structure and molecular interaction between material components induced specific properties to the substrate in terms of hydrophilicity degree, porosity and mechanical characteristics. Viability and proliferation assays demonstrated that these constructs allow adhesion and growth of mesenchymal stem cells (MSCs) and cardiac resident non myocytic cells (NMCs). Immunofluorescence analysis demonstrated that stem cells cultured on these constructs adopt a distribution mimicking the three-dimensional cell alignment of myocardium. qPCR and immunofluorescence analyses showed the ability of this construct to direct initial {MSC} and {NMC} lineage specification towards cardiomyogenesis: both {MSCs} and {NMCs} showed the expression of the cardiac transcription factor GATA-4, fundamental for early cardiac commitment. Moreover {NMCs} also acquired the expression of the cardiac transcription factors Nkx2.5 and {TBX5} and produced sarcomeric proteins. This work may represent a new approach to induce both resident and non-resident stem cells to cardiac commitment in a 3-D structure, without using additional stimuli.
date: 2014
date_type: published
publication: Biomaterials
volume: 35
number: 1
publisher: Elsevier
pagerange: 92 - 104
id_number: 10.1016/j.biomaterials.2013.09.058
refereed: TRUE
issn: 0142-9612
official_url: http://www.sciencedirect.com/science/article/pii/S0142961213011587
citation:   Cristallini, Caterina and Rocchietti, Elisa Cibrario and Accomasso, Lisa and Folino, Anna and Gallina, Clara and Muratori, Luisa and Pagliaro, Pasquale and Rastaldo, Raffaella and Raimondo, Stefania and Saviozzi, Silvia and Sprio, Andrea E. and Gagliardi, Mariacristina and Barbani, Niccoletta and Giachino, Claudia  The effect of bioartificial constructs that mimic myocardial structure and biomechanical properties on stem cell commitment towards cardiac lineage.  Biomaterials, 35 (1).  92 - 104.  ISSN 0142-9612      (2014)