relation: http://eprints.imtlucca.it/3251/
title: Surface functionalisation regulates polyamidoamine dendrimer toxicity on blood–brain barrier cells and the modulation of key inflammatory receptors on microglia
creator: Bertero, Alice
creator: Boni, Adriano
creator: Gemmi, Mauro
creator: Gagliardi, Mariacristina
creator: Bifone, Angelo
creator: Bardi, Giuseppe
subject: QD Chemistry
description: AbstractDendrimers are branched polymers with spherical morphology. Their tuneable chemistry and surface modification make them valuable nanomaterials for biomedical applications. In view of possible dendrimer uses as brain-aimed nanocarriers, the authors studied amine- and lipid-functionalised (G4) polyamidoamine (PAMAM) biocompatibility with cell population forming the blood–brain barrier (BBB). Both amine-PAMAM and lipid-PAMAM dendrimers were able to enter endothelial and primary neural cells. However, only amine-PAMAM damaged cell membranes in a dose-dependent manner. Transmission electron microscopy evidenced the ability of dendrimers to precipitate salts and serum components present in culture medium that slightly increased toxicity of the amine-PAMAM. Amine- and lipid-PAMAM were both able to cross the BBB and differently induced CD11b and CCR2 overexpression on primary CX3CR1-GFP murine microglia in vitro. These data emphasise the role of dendrimer surface functionalisation in toxicity and neural immune cell activation, raising concerns about possible neuroinflammatory reactions.
publisher: Taylor & Francis
date: 2014
type: Article
type: PeerReviewed
identifier:   Bertero, Alice and Boni, Adriano and Gemmi, Mauro and Gagliardi, Mariacristina and Bifone, Angelo and Bardi, Giuseppe  Surface functionalisation regulates polyamidoamine dendrimer toxicity on blood–brain barrier cells and the modulation of key inflammatory receptors on microglia.  Nanotoxicology, 8 (2).  pp. 158-168.  ISSN 1743-5390      (2014)  
relation: http://dx.doi.org/10.3109/17435390.2013.765054
relation: 10.3109/17435390.2013.765054