TY  - JOUR
KW  - Neuroinflammation; Glia; endothelial cells; polymer functionalisation; in vitro
AV  - none
TI  - Surface functionalisation regulates polyamidoamine dendrimer toxicity on blood?brain barrier cells and the modulation of key inflammatory receptors on microglia
UR  - http://dx.doi.org/10.3109/17435390.2013.765054
SN  - 1743-5390
N2  - AbstractDendrimers are branched polymers with spherical morphology. Their tuneable chemistry and surface modification make them valuable nanomaterials for biomedical applications. In view of possible dendrimer uses as brain-aimed nanocarriers, the authors studied amine- and lipid-functionalised (G4) polyamidoamine (PAMAM) biocompatibility with cell population forming the blood?brain barrier (BBB). Both amine-PAMAM and lipid-PAMAM dendrimers were able to enter endothelial and primary neural cells. However, only amine-PAMAM damaged cell membranes in a dose-dependent manner. Transmission electron microscopy evidenced the ability of dendrimers to precipitate salts and serum components present in culture medium that slightly increased toxicity of the amine-PAMAM. Amine- and lipid-PAMAM were both able to cross the BBB and differently induced CD11b and CCR2 overexpression on primary CX3CR1-GFP murine microglia in vitro. These data emphasise the role of dendrimer surface functionalisation in toxicity and neural immune cell activation, raising concerns about possible neuroinflammatory reactions.
ID  - eprints3251
EP  - 168
Y1  - 2014///
JF  - Nanotoxicology
IS  - 2
PB  - Taylor & Francis
A1  - Bertero, Alice
A1  - Boni, Adriano
A1  - Gemmi, Mauro
A1  - Gagliardi, Mariacristina
A1  - Bifone, Angelo
A1  - Bardi, Giuseppe
SP  - 158
VL  - 8
ER  -