eprintid: 3251
rev_number: 5
eprint_status: archive
userid: 69
dir: disk0/00/00/32/51
datestamp: 2016-03-21 09:38:57
lastmod: 2016-03-21 09:38:57
status_changed: 2016-03-21 09:38:57
type: article
metadata_visibility: show
creators_name: Bertero, Alice
creators_name: Boni, Adriano
creators_name: Gemmi, Mauro
creators_name: Gagliardi, Mariacristina
creators_name: Bifone, Angelo
creators_name: Bardi, Giuseppe
creators_id: 
creators_id: 
creators_id: 
creators_id: mariacristina.gagliardi@imtlucca.it
creators_id: 
creators_id: 
title: Surface functionalisation regulates polyamidoamine dendrimer toxicity on blood–brain barrier cells and the modulation of key inflammatory receptors on microglia
ispublished: pub
subjects: QD
divisions: CSA
full_text_status: none
keywords: Neuroinflammation; Glia; endothelial cells; polymer functionalisation; in vitro
abstract: AbstractDendrimers are branched polymers with spherical morphology. Their tuneable chemistry and surface modification make them valuable nanomaterials for biomedical applications. In view of possible dendrimer uses as brain-aimed nanocarriers, the authors studied amine- and lipid-functionalised (G4) polyamidoamine (PAMAM) biocompatibility with cell population forming the blood–brain barrier (BBB). Both amine-PAMAM and lipid-PAMAM dendrimers were able to enter endothelial and primary neural cells. However, only amine-PAMAM damaged cell membranes in a dose-dependent manner. Transmission electron microscopy evidenced the ability of dendrimers to precipitate salts and serum components present in culture medium that slightly increased toxicity of the amine-PAMAM. Amine- and lipid-PAMAM were both able to cross the BBB and differently induced CD11b and CCR2 overexpression on primary CX3CR1-GFP murine microglia in vitro. These data emphasise the role of dendrimer surface functionalisation in toxicity and neural immune cell activation, raising concerns about possible neuroinflammatory reactions.
date: 2014
date_type: completed
publication: Nanotoxicology
volume: 8
number: 2
publisher: Taylor & Francis
pagerange: 158-168
id_number: 10.3109/17435390.2013.765054
refereed: TRUE
issn: 1743-5390
official_url: http://dx.doi.org/10.3109/17435390.2013.765054
citation:   Bertero, Alice and Boni, Adriano and Gemmi, Mauro and Gagliardi, Mariacristina and Bifone, Angelo and Bardi, Giuseppe  Surface functionalisation regulates polyamidoamine dendrimer toxicity on blood–brain barrier cells and the modulation of key inflammatory receptors on microglia.  Nanotoxicology, 8 (2).  pp. 158-168.  ISSN 1743-5390      (2014)