eprintid: 816
rev_number: 17
eprint_status: archive
userid: 35
dir: disk0/00/00/08/16
datestamp: 2011-08-12 10:23:01
lastmod: 2013-03-05 15:47:20
status_changed: 2011-08-12 10:23:01
type: book_section
metadata_visibility: show
item_issues_count: 0
creators_name: Tsaftaris, Sotirios A.
creators_name: Ahuja, Ramandeep
creators_name: Shiell, Derek
creators_name: Katsaggelos, Aggelos K.
creators_id: sotirios.tsaftaris@imtlucca.it
creators_id: 
creators_id: 
creators_id: 
title: DNA microarray image intensity extraction using Eigenspots
ispublished: pub
subjects: QA76
subjects: QH426
divisions: CSA
full_text_status: none
keywords: DNA microarray image intensity extraction; Principal Component Analysis; background subtraction algorithms; eigenspots; eigenvectors; image analysis;l ocal background noise; organisms; DNA; eigenvalues and eigenfunctions; image processing; medical image processing; principal component analysis
abstract: DNA microarrays are commonly used in the rapid analysis of gene expression in organisms. Image analysis is used to measure the average intensity of circular image areas (spots), which correspond to the level of expression of the genes. A crucial aspect of image analysis is the estimation of the background noise. Currently, background subtraction algorithms are used to estimate the local background noise and subtract it from the signal. In this paper we use principal component analysis (PCA) to de-correlate the signal from the noise, by projecting each spot on the space of eigenvectors, which we term eigenspots. PCA is well suited for such application due to the structural nature of the images. To compare the proposed method with other background estimation methods we use the industry standard signal-to-noise metric xdev.
date: 2007
date_type: published
volume: 6
publisher: IEEE
pagerange: VI -265 
event_title: Image Processing, 2007. ICIP 2007. IEEE International Conference on
id_number: 10.1109/ICIP.2007.4379572
refereed: TRUE
isbn: 978-1-4244-1437-6 
book_title: International conference on image processing
official_url: http://ieeexplore.ieee.org/stamp/stamp.jsp?tp=&arnumber=4379572&isnumber=4379494
citation:   Tsaftaris, Sotirios A. and Ahuja, Ramandeep and Shiell, Derek and Katsaggelos, Aggelos K.  DNA microarray image intensity extraction using Eigenspots.   In:  International conference on image processing.   IEEE, VI -265 .  ISBN 978-1-4244-1437-6       (2007)