Cristallini, Caterina and Rocchietti, Elisa Cibrario and Accomasso, Lisa and Folino, Anna and Gallina, Clara and Muratori, Luisa and Pagliaro, Pasquale and Rastaldo, Raffaella and Raimondo, Stefania and Saviozzi, Silvia and Sprio, Andrea E. and Gagliardi, Mariacristina and Barbani, Niccoletta and Giachino, Claudia The effect of bioartificial constructs that mimic myocardial structure and biomechanical properties on stem cell commitment towards cardiac lineage. Biomaterials, 35 (1). 92 - 104. ISSN 0142-9612 (2014)
Full text not available from this repository.Abstract
Abstract Despite the enormous progress in the treatment of coronary artery diseases, they remain the most common cause of heart failure in the Western countries. New translational therapeutic approaches explore cardiomyogenic differentiation of various types of stem cells in combination with tissue-engineered scaffolds. In this study we fabricated PHBHV/gelatin constructs mimicking myocardial structural properties. Chemical structure and molecular interaction between material components induced specific properties to the substrate in terms of hydrophilicity degree, porosity and mechanical characteristics. Viability and proliferation assays demonstrated that these constructs allow adhesion and growth of mesenchymal stem cells (MSCs) and cardiac resident non myocytic cells (NMCs). Immunofluorescence analysis demonstrated that stem cells cultured on these constructs adopt a distribution mimicking the three-dimensional cell alignment of myocardium. qPCR and immunofluorescence analyses showed the ability of this construct to direct initial {MSC} and {NMC} lineage specification towards cardiomyogenesis: both {MSCs} and {NMCs} showed the expression of the cardiac transcription factor GATA-4, fundamental for early cardiac commitment. Moreover {NMCs} also acquired the expression of the cardiac transcription factors Nkx2.5 and {TBX5} and produced sarcomeric proteins. This work may represent a new approach to induce both resident and non-resident stem cells to cardiac commitment in a 3-D structure, without using additional stimuli.
| Item Type: | Article |
|---|---|
| Identification Number: | https://doi.org/10.1016/j.biomaterials.2013.09.058 |
| Uncontrolled Keywords: | Biocompatibility; Cardiac tissue engineering; Cell morphology; ECM (extracellular matrix); Scaffold; Stem cell |
| Subjects: | Q Science > QD Chemistry R Medicine > RZ Other systems of medicine |
| Research Area: | Computer Science and Applications |
| Depositing User: | Caterina Tangheroni |
| Date Deposited: | 21 Mar 2016 09:35 |
| Last Modified: | 21 Mar 2016 09:35 |
| URI: | http://eprints.imtlucca.it/id/eprint/3250 |
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