Bertero, Alice and Boni, Adriano and Gemmi, Mauro and Gagliardi, Mariacristina and Bifone, Angelo and Bardi, Giuseppe Surface functionalisation regulates polyamidoamine dendrimer toxicity on blood–brain barrier cells and the modulation of key inflammatory receptors on microglia. Nanotoxicology, 8 (2). pp. 158-168. ISSN 1743-5390 (2014)
Full text not available from this repository.Abstract
AbstractDendrimers are branched polymers with spherical morphology. Their tuneable chemistry and surface modification make them valuable nanomaterials for biomedical applications. In view of possible dendrimer uses as brain-aimed nanocarriers, the authors studied amine- and lipid-functionalised (G4) polyamidoamine (PAMAM) biocompatibility with cell population forming the blood–brain barrier (BBB). Both amine-PAMAM and lipid-PAMAM dendrimers were able to enter endothelial and primary neural cells. However, only amine-PAMAM damaged cell membranes in a dose-dependent manner. Transmission electron microscopy evidenced the ability of dendrimers to precipitate salts and serum components present in culture medium that slightly increased toxicity of the amine-PAMAM. Amine- and lipid-PAMAM were both able to cross the BBB and differently induced CD11b and CCR2 overexpression on primary CX3CR1-GFP murine microglia in vitro. These data emphasise the role of dendrimer surface functionalisation in toxicity and neural immune cell activation, raising concerns about possible neuroinflammatory reactions.
| Item Type: | Article |
|---|---|
| Identification Number: | 10.3109/17435390.2013.765054 |
| Uncontrolled Keywords: | Neuroinflammation; Glia; endothelial cells; polymer functionalisation; in vitro |
| Subjects: | Q Science > QD Chemistry |
| Research Area: | Computer Science and Applications |
| Depositing User: | Caterina Tangheroni |
| Date Deposited: | 21 Mar 2016 09:38 |
| Last Modified: | 21 Mar 2016 09:38 |
| URI: | http://eprints.imtlucca.it/id/eprint/3251 |
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